TCR diversity by high-throughput sequencing. (A) Chao2 estimates of total CDR3 rearrangements in PBMC samples. (B) CDR3 lengths for rearrangements involving V segment 12. (C) Linear regression of Chao2 estimates of integration site and CDR3 sequence total diversities. Dotted lines represent 95% confidence interval, and P value indicates probability that slope is zero.