Gene expression profiling of plerixafor and G-CSF–mobilized donor CD34⁺cells from leukapheresis products. (A,D) mRNA profiling was performed using Affymetrix U133+2 arrays on purified CD34⁺ stem cells obtained from 8 individual normal donors mobilized sequentially with plerixafor and G-CSF. (A) Shown are 22 genes with a raw signal intensity ≥400 (dotted line) in all the microarrays from 1 of the mobilizing agents; the ratio of plerixafor to G-CSF within each pair for the gene had to be at least a twofold difference all in the same direction. (B,E) mRNA profiling was performed using Affymetrix U133+2 arrays on purified CD34⁺ stem cells obtained from 4 individual normal donors mobilized with plerixafor or G-CSF. Statistical comparisons were performed using an unpaired 2-tailed Student t test. (C) GA-Mantel algorithm distance matrix of 22 original probes and 37 correlated probes. The dendrogram on the left is based on the 22 genes identified from the dual-threshold analysis approach; the dendrogram on the right is based on 37 genes found with a high Mantel correlation. (D) Expression of pDC lineage-associated genes in purified CD34⁺ stem cells obtained from 8 individual normal donors mobilized sequentially with plerixafor and G-CSF. Statistical comparisons were performed using a paired parametric Student t test. (E) Expression of pDC lineage-associated genes in purified CD34⁺ stem cells obtained from 4 individual normal donors mobilized with plerixafor or G-CSF. Statistical comparisons were performed using an unpaired 2-tailed Student t test. (F) Principal component analysis comparing CD34⁺ cells isolated from plerixafor or G-CSF–mobilized donors or normal bone marrow, which shows that the majority of CD34⁺ cells isolated from plerixafor-mobilized donors were distinct from the G-CSF–mobilized and normal bone marrow CD34⁺ cells. (G) Principal component analysis showing evident separation of CD34⁺CD45RA⁻ HSPCs, CD34^dimCD45RA⁺CD123⁺⁺ pro-DC2, and CD304⁺ pDCs isolated from plerixafor-mobilized donors. Statistical comparisons were performed using an unpaired 2-tailed Student t test. *P < .05, **P < .01, ***P < .001.