Analysis of PB parameters and colony formation assay. (A) PB parameters of control and Cbl^Q367P mice. A higher total WBC count was observed in Cbl^Q367P mice, which was mainly due to the proliferation of Mac1⁺ myelomonocytes, including Ly6C⁺⁺, CD43⁺ monocytic cells, and B220⁺ B lymphoid cells. (B) Giemsa-stained PB smears from control and Cbl^Q367P mice. The higher number of WBCs in the PB of Cbl^Q367P mice and higher magnification of WBCs with abnormal morphologies are shown in the upper and lower panels, respectively. Panels 1 and 2: WBCs with pseudo–Pelger-Huet anomaly and abnormal nuclei (indicated by arrows); panel 3: a hypersegmented neutrophil (indicated by an arrow); and panels 4–6: giant platelets, an erythrocyte with a Howell-Jolly body, and an apoptotic cell (indicated by arrows, an arrowhead, and a white arrowhead, respectively). (C) Changes of PB parameters during the observation period. Cbl^Q367P mice exhibited a sustained elevation of WBC numbers after pIpC stimulation. (D) Hematopoietic colony numbers of control and Cbl^Q367P mice. Cbl^Q367P hematopoietic cells exhibited a significant increase in total, mix, and GM colonies and generated a remarkable number of spontaneous colonies. ***P < .001; **P < .01; *P < .05. ns, not significant.