Forced expression of Gem in HSPCs. (A) Experimental procedure. The c-kit⁺ cells of control or Cbl^Q367P mice (Ly5.1⁺) were infected with Gem-IRES-EGFP–expressing retrovirus, and sorted EGFP-positive cells were subjected to in vitro culture or BMT into recipient mice (Ly5.2⁺). (B) Results of the in vitro culture. Representative plots of flow cytometry and percentages of the LSK and Mac1⁺, Gr1⁺ cells are shown in the left and right panels, respectively. (C) Results of BMT. The changes of percentages of the Ly5.1⁺ cells in the PB, percentages of lineage marker–positive Ly5.1⁺ cells in the PB, and percentages of total, LSK, and Mac1⁺ Ly5.1⁺ cells in the BM at the end point of BMT are shown in the left, middle, and right panels, respectively. *P < .05. ns, not significant.