Shifts in microbial communities to WT mice after cohousing with IL-17RA^−/−or IL-17RC^−/−mice are similar. (A) Heatmap displaying differentially abundant OTUs consistently increased or decreased in WT mice after cohousing with IL-17RA^−/− or IL-17RC^−/− mice for 4 weeks. Heatmap generated based on fecal microbiota profile from 16S rRNA sequencing of WT, IL-17RA^−/−, and IL-17RC^−/− mice. Each column represents an individual WT mouse. Data from 2 IL-17RA^−/− cohousing experiments are shown (n = 12 mice, experiments 1 and 2) and 1 IL-17RC^−/− cohousing experiment (n = 10 mice, experiment 3). Scale is based on normalized read counts generated using the regularized log transformation implemented within DESeq2. Details available in supplemental Table 3. (B) Representative fluorescence in situ hybridization images of the colon at day 6 post-alloSCT (WT [left] and IL-17RA^−/− [right]) utilizing universal rRNA probes to show bacteria (red) and their translocation from the lumen (L) into the tissue (yellow arrows). Bacterial morphology is highlighted in the higher magnification image shown of the boxed area (red outline). DAPI nuclear stain (blue). Scale bar = 50 μm. MM, muscularis mucosae; SM, submucosa.