Both hematopoietic and nonhematopoietic cell DREAMs participate in thrombosis and hemostasis, and platelet DREAM is important for thrombogenesis. WT or DREAM KO transplant control mice and DREAM bone marrow chimeras (hematopoietic [HP] or non-HP DREAM KO) were used for intravital microscopy as described in Figure 1. (A) Representative images. Arrows show direction of blood flow. (B-C) F platelet and fluorescence intensities of anti-CD42c antibodies in 4 different groups are presented as described in Figure 1 (32-38 thrombi in 5 mice of each group). **P < .01 and ***P < .001 vs WT control mice and #P < .05, ##P < .01, and ###P < .001 between 2 groups after the Mann-Whitney U test. (D) Cremaster arteriolar thrombosis was induced by FeCl₃ injury. Time to occlusion was plotted (n = 8-10). (E-F) Tail bleeding time and blood loss were measured as described in Figure 1D-E (n = 8). (G-I) Fluorescently labeled WT or DREAM KO platelets, 10⁸ in 100 µL of saline, were infused into thrombocytopenic WT mice. Three thrombi were generated following laser injury. The same labeled platelets were reinfused into the mouse to generate 3 additional thrombi. Accumulation of infused platelets was monitored and analyzed as described above. Horizontal bars represent the median value of the fluorescence intensities of anti-CD42c antibodies (C,I), time to occlusion (D), bleeding times (E), or Hb content (F). *P < .05 and ***P < .001 vs WT platelets after the Mann-Whitney U test (n = 4-5 mice for each group) or after the Dunn test (D-F).