miR-155 promotes colony-forming potential and represses Cebpb and Stat1 expression in primary FLT3-ITD⁺AML cells. (A) miR-155 expression in primary FLT3-ITD⁺ AML samples treated with a CTRL LNA or miR-155 LNA determined by qRT-PCR. Expression normalized to 5S ribosomal RNA. (B) Colony-forming potential of primary FLT3-ITD⁺ AML samples grown in methylcellulose agar supplemented with 100 nM CTRL LNA or miR-155 LNA. (C) Annexin V staining of primary FLT3-ITD⁺ AML samples treated with CTRL LNA or miR-155 LNA for 3 days in liquid culture. (D) qRT-PCR analysis of primary FLT3-ITD⁺ AML samples treated with CTRL LNA or miR-155 LNA. Expression normalized to L32, with LNA-CTRL treated cell average set to a relative expression of 1. Each point represents an individual patient sample. *P ≤ .05; **P ≤ .01.