Hbb^th3/+ mouse neutrophils display impaired ROS activity. Whole cell populations from (A) BM, (B) blood, and (C) spleen were incubated with opsonized, heat-killed S aureus. At indicated time points, the ROS production was measured using 2′,7′-dichlorofluorescein as an indicator, and expression of CD11b⁺ and Ly6G^high to identify the neutrophils in each sample. Statistical significance between WT and Hbb^th3/+ mice was evaluated using 2-way ANOVA. Data are presented as mean ± SEM, n ≥ 3 per mice group.