Hbb^th3/+ mice have an increased number of immature neutrophils in the spleen. (A) Representative dot plot analysis of CD11b⁺CD49b^neg cells showing (i) neutrophils (Ly6G^highLy6C^low), (ii) immature myeloid cells (Ly6G^lowLy6C^low), and (iii) monocytes (Ly6G^negLy6C^high) in the spleen of WT and Hbb^th3/+ mice. Morphological analysis of Ly6G^highLy6C^low neutrophils from the spleen of (B) WT and (C) Hbb^th3/+ mice, stained with May-Grünwald-Giemsa stain (original magnification ×400; enlarged insets digitally expanded from the ×400 images). (D) Percentage of band form and segmented cells among the sorted Ly6G^highLy6C^low neutrophils in the spleen of WT and Hbb^th3/+ mice. Results are presented as mean and SEM of n = 5. (E) Morphological analysis of immature myeloid cells sorted from the spleen of Hbb^th3/+ mice and stained with May-Grünwald-Giemsa stain (original magnification ×400; enlarged inset digitally expanded from the ×400 image). Relative abundance of (F) neutrophils (Ly6G^highLy6C^low), (G) immature myeloid cells (Ly6G^lowLy6C^low), and (H) monocytes (Ly6G⁻Ly6C^hi) in BM, blood, and spleen of WT and Hbb^th3/+ mice (among CD11b⁺CD49b^neg cells). Shown are results from individual animals, with horizontal line indicating mean for each group. The statistical significance between the groups was evaluated with Student t test.