Pathways show differential patterns of phospho-regulation. Per time point, regulated phosphoproteins were analyzed for enriched GO-terms, which mainly show a similar trend: (1) strong regulation after 10 s, (2) followed by a clearly reduced regulation over time. The pathways “transmembrane receptor protein tyrosine kinase signaling” and “enzyme linked receptor protein signaling” clearly deviated from this pattern, showing a second strong phase of regulation at 60 seconds.