Figure 1.
Dex regulates B-cell development genes in sensitive B-ALL samples. (A) Heatmap clustering genes commonly regulated (KS test, q ≤ 10−4) by dex across 16 samples. Primary and PDX samples are marked red; cell lines, black. (B) Ingenuity pathway analysis of regulated genes shows enrichment for hematological development genes. (C) Stop or push through model for dex in B-cell development highlighting the roles of dex-repressed ITGA4, IL7R, and BCL6. (D-F) Differential gene expression values across sensitive B-ALL sample across samples measured by microarray (left) and GR occupancy in sensitive (B1) and resistant (HM3101) samples measured by ChIP-seq in response to dex suggest ITGA4, IL7R, and BCL6 are direct targets of GR regulation.
