Kinetics of white blood cells and neutrophil subsets after LPS injection. (A) Neutrophil counts were decreased 1 hour after LPS injection (t = 0), followed by increased counts. (B) Counts of other leukocytes were also decreased at t = 1 hour, but most had recovered by t = 6 or 8 hours. (C) Different neutrophil subsets were identified (t = 3 hours) based on CD16 and CD62L expression. (D) The appearance of the banded and CD62L^dim subsets was followed over time. (E) After sorting, microscopic analysis of the different subsets isolated at t = 3 hours post–LPS challenge revealed an increasing nuclear lobularity from banded to segmented to CD62L^dim neutrophils. Symbols and bars represent mean ± 95% CI (n = 20) (A,B,D) or medians ± interquartile range (n = 7-8) (E). *P < .05, **P < .01, ***P < .001, and ****P < .0001 compared with t = 0 hours, as determined by repeated-measures 1-way analysis of variance with Dunnett’s or Holm-Sidak correction for multiplicity where applicable.