Label kinetics in plasma and in DNA of neutrophil subsets. Healthy volunteers were labeled with 6,6²H₂-glucose over the course of 6 hours. (A) During this period, the availability of label was monitored by measuring the fraction of 6,6-²H₂-glucose in the total blood glucose pool in blood samples obtained by skin pricks. (B) DNA ²H enrichment of the different inflammatory neutrophil subsets 3 hours after LPS injection or total neutrophils isolated 5 minutes pre-LPS injection at 3 to 11 days after 6,6-²H₂-glucose. Results suggest that banded neutrophils are less mature than the 2 other subsets. (A) Fraction enrichment was corrected for availability of label for each individual. Symbols indicate mean ± standard deviation, with (A) n = 20 and (B) n = 4 volunteers at each time point, with a total of 20 volunteers. *P < .05 for banded compared with segmented neutrophils, #P < .05 for banded neutrophils compared with neutrophils isolated before LPS injection, and ***P < .001 for banded neutrophils compared with all 3 other subsets as determined by a repeated-measures 2-way analysis of variance with Tukey’s correction for multiplicity.