Effect of Plg/Pla on in vivo peritoneal macrophage efferocytic capacity. Mice received an i.p. injection of 0.1 mg of zymosan and then were injected i.p. with Plg (2 µg per cavity), Pla (2 µg per cavity), or vehicle 62 hours later. Seven hours after treatments, mice were injected i.p. with 106 apoptotic neutrophils. Mice were killed 3 hours later. (A) Efferocytosis was assessed on cytospin preparations of cells harvested from peritoneal lavage after staining with May-Grünwald-Giemsa. Representative figures are shown in (B). Original magnification ×40. In the same experimental settings, 2 µg of Pla was incubated with 11.2 µg of VPLCK or 3.5 mg of EACA for 1 hour at 37°C prior to i.p. injection (C). Results are presented as the mean efferocytosis index ± SEM. The experiments were performed 3 times with at least 5 mice in each group. *P < .05; **P < .01 when compared with untreated mice; #P < .05 when compared with Pla-treated mice.
