Analysis of FLI1 mRNA and protein levels of FLI1, MPL, and PF4. Day 5 iMegs were selected for CD41a and analyzed using quantitative reverse transcription polymerase chain reaction and western blot. (A) Relative expression of FLI1 mRNA was performed using quantitative reverse transcription polymerase chain reaction compared with WT. Mean ± 1 SEM are shown of 4 separate experiments. P values were calculated using 1-way ANOVA. (B) Western blot analysis of FLI1. Relative expression was compared with WT. Means ± 1 SEM are shown for 4 separate experiments. P values were calculated using 1-way ANOVA. (C) Flow cytometric analysis of MPL. Relative surface MPL expression analyzed from 3 separate experiments using flow cytometry and 1-way ANOVA. (D) Similar to panel B, but measuring PF4.