Megacult colony assay and iMeg differentiation in liquid culture. (A) HPCs were plated in a semisolid Megacult colony system and analyzed at the end of the assay for colony count per input HPC. Means ± 1 SEM are shown along with the number of independent experiments performed. Significant P values performed using 1-way ANOVA are shown. (B) HPCs were grown in liquid culture and analyzed at the end of the assay for iMeg numbers. Relative expression to WT iPSC line is shown. Means ± 1 SEM are shown, along with the number of independent experiments performed. P values were calculated using 1-way ANOVA.