Different GF combinations activate different signaling responses in CD49f⁺ CB cells. (A) Experimental design for cells examined (B-D). (B) Representative histograms of signal intensity over time for pERK1/2 (top), pSTAT3 (middle), and active (nonphosphorylated) β-catenin (bottom) following stimulation of CD49f⁺ cells with 5 GFs. Histogram colors indicate the median shift in the stimulated cells relative to the unstimulated cells. The color scale is indicated by the color bar in panel C. (C) Median asinh (signal intensity/5) differences in signal activation in the CD49f⁺ cells compared with unstimulated cells. Significant differences are indicated in green. (D) Frequency of CD49f⁺ cells surpassing the 95th percentile of the unstimulated sample in any intracellular marker following GF stimulation. (E) Signal activation shown as differences in median asinh values measured in CD49f⁺ cells exposed to either SCF + FLT3L + TPO or SCF + FLT3L + TPO + IL-6 for the times shown (in minutes) after a preliminary hour in SFM alone relative to CD49f⁺ cells incubated in SFM for equivalent times without GFs.