Subsets within the CD49f⁺ compartment exhibit different molecular responses to GFs. Dimensionality reduction using t-SNE was performed on the data obtained for all intracellular markers from all CD49f⁺ cells after scaling the data from each CyTOF run. Subsets of the CD49f⁺ compartment are shown as different colors and their frequencies are listed in the figure. Cells that fell in between gates are not shown. (A) The distribution of unstimulated (unstim) cells from all subsets in t-SNE space. (B) The overall distributions of all unstimulated cells are shown as contours on all plots. Results for each subset of CD49f⁺ cells following the indicated GF exposure are shown in t-SNE space.