GSK’872 and GSK’843 each inhibits phosphatidylserine exposure in PMN-SA. PMN were pretreated without or with 50 μM GSK’872 or 50 μM GSK’843 and then left in buffer alone or challenged with SA, as indicated in “Methods.” After 3 hours, PMN were stained with Annexin V-FITC and PI to detect surface exposure of phosphatidylserine and to assess membrane integrity, respectively, and analyzed by flow cytometry. Bars represent the mean of 3 separate experiments ± SEM. P values were determined using repeated measures 1-way ANOVA and Dunnett posttest (*P < .05 vs SA).