MYC expression in MCL. (A) Western blot analysis of MYC expression in 10 MCL cell lines and in the MM cell line U266. All MCL cell lines had detectable MYC expression compared with the negative control U266. (B) Immunohistochemical MYC staining of an MYC-positive MCL case (left: original magnification ×400) and an MYC-negative MCL case (right: original magnification ×400). Images were captured using an Olympus BX51 microscope (Olympus, Tokyo, Japan) equipped with an Olympus DP73 camera and were processed with Olympus cellSens software. (C) Frequency of MYC expression in MCL. (D) Mean MYC expression in cytological MCL variants. MYC expression was significantly higher in pleomorphic and blastoid variants compared with classical MCLs. Error bars indicate the standard error of the mean.