Angiocrine Bmp2 signaling in the liver controls tissue and serum iron concentrations via regulation of hepatocyte hepcidin expression. (A-E) Iron concentration in (A) liver, (B) serum, (C) spleen, (D) heart, and (E) pancreas of Bmp2^LSECKO and control mice (female, n = 5; male, n = 5). *P < .05; **P < .01; ****P < .0001. (F) qRT-PCR of Hamp, Id1, Id2, Id3, Endoglin (Eng), and Atoh8 in liver of Bmp2^LSECKO and control mice (female, n = 5; male, n = 5). β-Actin was used as housekeeping gene. *P < .05; ***P < .001; ****P < .0001. (G) Serum hepcidin levels of Bmp2^LSECKO and control mice as measured by enzyme-linked immunosorbent assay (female, n = 5; male, n = 5). ***P < .001. (H) Gene expression heat map of murine hepatocytes stimulated with Bmp2 for 24 hours (left panel). Bmp2-dependent expression of Hamp, Id1, Id2, Id3, and Atoh8 was quantified by qRT-PCR (right panel) of hepatocytes stimulated with Bmp2 for 24 hours and of murine LSECs (mLSECs) stimulated with Bmp2 for 48 hours. β-Actin was used as housekeeping gene. *P < .05; **P < .01; ***P < .001; ****P < .0001.