Detection of HLA-B4002-lacking granulocytes using an anti-HLA-B61 mAb in AA patients with HLA-B*40:02. (A) Representative dot plots of 3 patients with HLA-A2, A24, and B4002 are shown. (i) Case 22, which had only wild-type, B4002⁺A24⁺A2⁺ cells. (ii) Case 12, which showed B4002⁻ cells that lacked HLA-B4002 but retained both HLA-A alleles (A24 and A2), designated as B4002⁻A24⁺A2⁺, in addition to 6pLOH(+) cells lacking both HLA-B4002 and A24 on the same haplotype. (iii) Case 7, which showed B4002⁻A24⁺A2⁺ cells but did not show either B4002⁻A24⁻ or B4002⁻A2⁻ (6pLOH[+]) cells. (B) Four different patterns of HLA-B4002-lacking cell status and their proportions in 28 AA patients with HLA-B*40:02. FITC, fluorescein isothiocyanate; PE, phycoerythrin.