Characterization of CD247 revertants. (A) Freshly isolated CD247^−/− patient PBMCs were stained, and rare CD247 revertant NK cells were observed. (B-C) Mitogen- and antigen receptor–stimulated PBMCs from a patient were analyzed by flow cytometry (B) and western blot (C) for TCR and CD247 expression. (D-E) Somatic mutants recovering CD247 expression were sequenced (D), and their ability to restore surface TCR expression was analyzed using the CD247-deficient Ma5.8 cell line (E). *P < .05; **P < .01; ***P < .001; n.s. (not significant), P > .05. ITAM, immunoreceptor tyrosine-based activation motif; PHA, phytohemagglutinin; TM, transmembrane; WT, wild-type.