shRNA screens for genes uniquely required by AML cell lines for survival. (A) Schematic representation of data analysis workflow. One hundred ninety-seven genes with ≥2 significant shRNAs were selected, and 214 genes with significant ATARiS scores were selected. The 2 lists have 58 overlapping genes and 353 genes in total. (B) The 353 screen hits are enriched of genes highly expressed in CCLE AML cell lines based on GSEA.³⁰  (C) Examples of screen hits that are frequently altered in AML or required by AML cells for survival compared with non-AML cancer cell lines. Lines indicate mean. FDR, false discovery rate; NES, normalized enrichment score.