Upregulation of FOXO1 depends on the presence of AE. qPCR and immunoblot analysis to determine FOXO1 mRNA (A; n = 3, error bars represent SD) and protein (B) in AE-tet-off system following doxycycline (DOX) addition. (C and D) RNA-seq analysis of FOXO1 expression of Kasumi-1 cells transected with control siRNA (siMM) or AE siRNA (siAE). RNA-seq tracks (C) and relative fragments per kilobase of transcript per million mapped reads (FPKM) values (D) are shown. n = 3; error bars represent SD. P value was calculated by unpaired 2-tailed Student t test. (E) ChIP-seq analysis showed AE binding patterns at FOXO1 locus in Kasumi-1 cells. The sequence of the binding peak is shown with RUNX1 binding motifs highlighted. (F) ChIP-qPCR analysis of chromatin occupancy of AE (HA-tagged) at FOXO1 locus in AE cells, with primers indicated in panel E by arrow. RPL30 locus was used as negative control. n = 3; error bars represent SD. IgG, immunoglobulin G.