FOXO1 regulates AE key target genes. (A) Genomic location of AE and FOXO1 binding sites. (B) Venn diagram showing the overlap between AE and FOXO1 ChIP-seq peaks. (C) Enriched transcription factor binding motifs in jointly bound AE and FOXO1 ChIP-seq peaks. (D) Percentage of peaks containing predicted enriched motifs. (E) Percentage of AE-regulated genes associated with both AE and FOXO1 binding. (F) ChIP-seq tracks at SOX4, MPL, and UBASH3B loci showing binding patterns of AE and FOXO1 in AE cells, H3K9Ac in Kasumi-1 cells, and layered H3K27Ac peaks of 7 cell lines from ENCODE. Arrows indicate jointly bound AE and FOXO1 peaks. (G) Immunoblot to determine protein levels of MPL, UBASH3B, and SOX4 upon FOXO1 knockdown in AE cells.