Histone gene mutations co-occur within individual patients with FL. Coding and splice site mutations in genes encoding the core histones (H2A, H2B, H3, H4) or histone linker (H1) often co-occur within patients. Each row represents a mutated histone gene, and each column represents a patient in this cohort. Histone mutations per patient are displayed at the top, indicating the total number of genes mutated (also summarized for the cohort in the bar graph on the left) and total number of mutations observed (includes multiple mutations per gene). The distribution of mutations and mutation types are indicated by colored boxes in the grid. For genes with multiple mutations in a single patient, only 1 mutation type is shown, with priority order indicated in the legend from the highest priority at the left to lowest at the right. Visualization created using GenVisR.41 (Inset) Histogram depicts the distribution of expected total histone gene mutation co-occurrences from 10 000 randomly permutated datasets with respect to the observed total co-occurrence in this cohort indicated by a red line (estimated P value < .0001). Although some patients had more than 1 mutation per histone gene, as indicated at the top, genes were considered mutated or not mutated for co-occurrence analysis. See supplemental Table 11 for a complete list of mutations. FS, frame shift; IF, in frame; SS, splice site.
