Evolution of clonal architecture during the natural course of the disease. Clonal architecture on the basis of single CD34⁺CD38⁻ cells by the Sanger method. The proportions of each subclone are indicated as percentages and 95% CIs. Light blue circles and bold type represent the dominant subclone statistically identified using the Fisher exact test. (A) UPN 8 at diagnosis and after 30 months. (B) UPN 3 at diagnosis and after 69, 90, and 113 months.