Figure 1.
Figure 1. Reduced GC response in IgH-hEBI2 mice. (A). Gating scheme used to define the splenic CD19+IgDlowFas+GL7+ GC B-cell population. (B) Subsets of splenic GC B cells of young (13 weeks) WT and IgH-hEBI2 mice were analyzed by FACS as shown in panel A. Cells were gated on lymphocytes. The numbers of GC B cells are given as percentage of total lymphocytes. Data are mean ± standard error of the mean (SEM) of data from 5 to 6 mice. *P < .05 by the Student t test. (C). IgH-hEBI2 and WT mice were immunized with DNP-OVA precipitated in alum followed by boosters 21 and 42 days (d) later. The concentration of serum anti-DNP-OVA IgG1 in the mice was determined by ELISA in serum samples taken 2 weeks after the indicated time points. The results are mean ± SEM of data from 10 IgH-hEBI2 and 7 WT mice. P values for log 50% inhibitory concentration (IC50) values are .0037 and <.0001 for serum concentrations after the first immunization and the first boost, respectively. (D). IgH-hEBI2 and WT mice were immunized with DNP-OVA precipitated in alum. The concentration of serum anti-DNP-OVA IgM was determined by ELISA in serum samples taken 2 weeks after immunization. The results are mean ± SEM of data from 10 IgH-hEBI2 and 7 WT immunized mice and 3 nonimmunized control mice. (E). qPCR analysis of BCL-6 expression in spleen cells from 12- to 15-week-old IgH-hEBI2 and WT mice relative to the expression of the control gene, GAPDH. The results are mean ± SEM of data from 3 mice. *P < .05 by nonparametric Mann-Whitney test. (F). qPCR analysis of AID expression in spleen cells from 12- to 15-week-old IgH-hEBI2 and WT mice relative to the expression of the control gene, GAPDH. The results are mean ± SEM of data from 5 mice. **P < .01 by nonparametric Mann-Whitney test. (G). Gating scheme used to define splenic B220+CD38+ memory B cells in DNP-OVA immunized mice. (H). Number of memory B cells in spleens of DNP-OVA immunized WT and IgH-hEBI2. Mice were euthanized 2 weeks after the second booster immunization and spleens were used for FACS. The number of memory cells is given as percentage of total lymphocytes. The results are mean ± SEM of data from 10 IgH-hEBI2 and 7 WT mice. *P < .05 by Student t test. AID, activation-induced cytidine deaminase; SSC, side scatter.

Reduced GC response in IgH-hEBI2 mice. (A). Gating scheme used to define the splenic CD19+IgDlowFas+GL7+ GC B-cell population. (B) Subsets of splenic GC B cells of young (13 weeks) WT and IgH-hEBI2 mice were analyzed by FACS as shown in panel A. Cells were gated on lymphocytes. The numbers of GC B cells are given as percentage of total lymphocytes. Data are mean ± standard error of the mean (SEM) of data from 5 to 6 mice. *P < .05 by the Student t test. (C). IgH-hEBI2 and WT mice were immunized with DNP-OVA precipitated in alum followed by boosters 21 and 42 days (d) later. The concentration of serum anti-DNP-OVA IgG1 in the mice was determined by ELISA in serum samples taken 2 weeks after the indicated time points. The results are mean ± SEM of data from 10 IgH-hEBI2 and 7 WT mice. P values for log 50% inhibitory concentration (IC50) values are .0037 and <.0001 for serum concentrations after the first immunization and the first boost, respectively. (D). IgH-hEBI2 and WT mice were immunized with DNP-OVA precipitated in alum. The concentration of serum anti-DNP-OVA IgM was determined by ELISA in serum samples taken 2 weeks after immunization. The results are mean ± SEM of data from 10 IgH-hEBI2 and 7 WT immunized mice and 3 nonimmunized control mice. (E). qPCR analysis of BCL-6 expression in spleen cells from 12- to 15-week-old IgH-hEBI2 and WT mice relative to the expression of the control gene, GAPDH. The results are mean ± SEM of data from 3 mice. *P < .05 by nonparametric Mann-Whitney test. (F). qPCR analysis of AID expression in spleen cells from 12- to 15-week-old IgH-hEBI2 and WT mice relative to the expression of the control gene, GAPDH. The results are mean ± SEM of data from 5 mice. **P < .01 by nonparametric Mann-Whitney test. (G). Gating scheme used to define splenic B220+CD38+ memory B cells in DNP-OVA immunized mice. (H). Number of memory B cells in spleens of DNP-OVA immunized WT and IgH-hEBI2. Mice were euthanized 2 weeks after the second booster immunization and spleens were used for FACS. The number of memory cells is given as percentage of total lymphocytes. The results are mean ± SEM of data from 10 IgH-hEBI2 and 7 WT mice. *P < .05 by Student t test. AID, activation-induced cytidine deaminase; SSC, side scatter.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal