Lymphoma cells from IgH-hEBI2 animals can be transferred to and survive in nude mice. (A) Picture of a B6-ν/ν mouse injected with lymphoma cells from a 16-month-old IgH-hEBI2 mouse. The picture is representative of mice injected with lymphoma spleen cells from IgH-hEBI2 mice. (B) Spleen weights of B6-ν/ν mice injected with spleen cells from age-matched control WT mice and lymphoma IgH-hEBI2 mice. The results are data from 10 to 14 mice. ****P < .0001 by Student t test. (C) Representative plot of surface expression of B220 and CD19 on spleen cells from B6 nude mice injected with lymphoma cells from IgH-hEBI2 mice and spleen cells from WT mice and quantification of CD19⁺/B220^hi, CD19⁺/B220^low, and CD19⁺/B220⁻ B-cell subsets as determined by FACS. (D) Representative histologic section from an B6-ν/ν mouse injected with spleen cells from a IgH-hEBI2 lymphomatic mice. The sample is stained with H&E or PAX5 (B-cell marker) (original magnification ×400).