Zeb2 deficiency alters hematopoietic stem and progenitor compartment. (A) Representative immunophenotypic analysis and gating strategy of control and Zeb2^Δ/ΔMx1-Cre hematopoietic stem and progenitor populations at 8 weeks after Poly(I:C) injection. (B) Significant increase of HSPCs (defined as lineage⁻cKit⁺Sca1⁺ [LKS]) and HSC (LKS-CD48⁻CD150⁺ [LKS-SLAM]) populations in the absence of Zeb2 compared with control BM. (C) Analyses of lineage restricted progenitor subsets (lineage⁻cKit⁺Sca1⁻) revealed a shift toward more MEP and less granulocyte-monocyte progenitor (GMP) subsets in Zeb2-deficient hematopoietic compartment, whereas no difference in the appearance of CLP and CMP populations was detected. Data are representative of 3 independent experiments using 6 to 7 mice. Percentages refer to all nucleated BM cell and mean ± standard error of the mean (SEM) is shown. CLP, lineage⁻CD127⁺cKit^dimSca1^dim; CMP, lineage⁻cKit⁺Sca1⁻CD34⁺CD16/32⁻. Unpaired, 2-tailed t test was performed to determine significance; *P < .05, **P < .01, ***P < .001.