Distinct chemokine receptor profile of CD4⁺CD161⁺Rho^loT cells. (A) Histogram comparing the MFI of CCR7 among CD4⁺CD161⁺Rho^lo (red), CD4⁺CD161⁺Rho^hi (blue), and CD4⁺CD161⁻ (green) T cells. (B) Representative FACS plots depicting the chemokine receptor profile, including CCR6, CCR5 and CXCR7 expression, on CD4⁺CD161⁺Rho^lo (left), CD4⁺CD161⁺Rho^hi (middle), and CD4⁺CD161⁻ (right) T cells; cumulative results from 5 independent experiments are shown in C. Each dot represents 1 individual. (D) Representative FACS plot depicting expression of CCR6, CCR4, and CXCR3 on CD4⁺CD161⁺Rho^lo (lower left) and CD4⁺CD161⁺Rho^hi (lower right) T cells. (E) FACS-purified CD161⁺Rho^lo (top), CD161⁺Rho^hi (middle), and CD161⁻ (bottom) CD4⁺ T cells were stimulated with phorbol 12-myristate 13-acetate/ionomycin in the presence of brefeldin A for 6 hours. Indicated cytokines were analyzed by intracellular staining. (F) Dot plots comparing cytokine production among T-cell subsets with a low-, high-, or no-efflux capacity. **P < .01; ***P < .001; ****P < .0001.