Loss of active histone marks at hypermethylated sites. (A) Hypermethylated DMCs show reduced enrichment of active marks H3K4me1, H3K4me3, and H3K27ac in AML (red) at both TSS proximal and TSS distal DMCs relative to CD34⁺ cells (blue). (B) Hypermethylated TSS distal DMCs within and adjacent to the CACNA2D2 gene locus. Loss of H3K4 methylation and H3K27 acetylation is observed in AML samples at the sites of hypermethylation.