Impaired ovary functions and fertility in GVHD. Mice were transplanted as in Figure 1A-D. (A) Immunofluorescent staining for AMH (red) with DAPI (blue) counter staining. Original magnification ×20. Scale bar, 100 µm. (B) Serum levels of AMH of naive (n = 6), syngeneic (n = 6), and allogeneic (n = 13) recipients on day +21 were shown as means ± SE. (C) Numbers of oocytes ovulated on stimulation with PMSG and hCG. Data from a representative of 2 similar experiments are shown as means ± SE. (D-F) Syngeneic (n = 6) and allogeneic (n = 9) recipients were mated with naive males repeatedly from day +14 to day +100 after SCT. Total numbers of newborns delivered by day +100 (D), numbers of newborns per litter (E), and body weight of newborns (F) are shown as means ± SE. **P < .01, ***P < .005.