Phenotypic discrepancies between circulating CD158k⁺T cells and skin-derived SCs. (A) Naïve/memory phenotypes, in favor of more mature subtypes in skin-derived SCs (n = 16). (B) Example of characterization of skin-derived Vβ22⁺CD158k⁺ T cells from patient MOTM35, showing the exclusively memory TTM/TEM phenotypes (CD45RA⁻CCR7⁻CD27^±CD95⁺). These SCs express CD69, a marker of TRM, but not CD103. Ninety-four percent of the CD158k⁺ T cells coexpress TCRVβ22. (C) Expression CXCR3, CCR6, CCR10 (n = 16), and CCR4 (n = 13). (D) Expression of CD127 (IL-7Rα, n = 9), CD215 (IL-15Rα, n = 8), and CD25 (IL-2Rα, n = 16). Results were obtained from immunostaining on fresh whole blood and extracted cutaneous cells from 16 patients. Statistical analyses are paired among patients (Wilcoxon test). Medians ± interquartile ranges are indicated.