Pan-HDAC inhibitors upregulate CD20 levels. Raji cells were preincubated for 48 hours with increasing concentrations of pan-HDAC inhibitors trichostatin A, SAHA, and scriptaid. (A) The levels of surface CD20 were analyzed with flow cytometry on staining with FITC-conjugated anti-CD20 antibody. Cell viability and statistical analysis were performed as described earlier (Figure 1A). The results are presented as a percentage of MFI of control cells (± SD). Statistical significance was determined with Kruskal-Wallis test, **P < .01 vs controls. The experiments were repeated independently 3 times. (B) cDNA from Raji cells preincubated for 24 hours with HDAC pan-inhibitors was used for qRT-PCR amplification of CD20 and β2M with corresponding probes labeled with FAM and DABCYL. Relative reverse transcription quantitative polymerase chain reaction expression of CD20 gene was calculated by the user noninfluent second derivative method and shown as log-transformed target to reference ratio. B2M gene served as reference. The statistical significance was assessed using unmatched pairs t-test. *P < .05 vs controls. The experiments were repeated independently twice.