T-cell responses to LAAs PRAME, WT1, BMI-1, and PR3 in CML patients at diagnosis, MMR, MR^4.5, and in HD. (A) CTL-mediated immune responses to LAAs in CML patients at diagnosis (n = 10), MMR (n = 15), and MR^4.5 (n = 19) and in HD (n = 7). (B) CTL-mediated immune responses to HLA-A*0201–restricted peptides in CML patients at diagnosis (n = 5), MMR (n = 7), and MR^4.5 (n = 12). (C) Representative IFN-γ ELISPOT showing T-cell response to PRAME in the same patient at diagnosis (no response, left) and MMR (positive response with IFN-γ production, right). Each colored spot represents a single IFN-γ–secreting cell. SEB, staphylococcal enterotoxin B; TNTC, too numerous to count. Images were captured and IFN-γ spot counts were assessed using AID iSpot Flurospot Reader System. (D) CTL-mediated immune responses to CMV in CMV-positive patients at diagnosis (n = 4), MMR (n = 6), and MR^4.5 (n = 10). Columns represent the mean number of CMV-specific spots/1 × 10⁵ cells.