Mitochondria transfer to leukemic blasts occurs via TNT. (A) BMSC were transduced with a rLV.EF1. mCherry-Mito-9 Lentivirus. AML blasts were cultured on mCherry-Mito-9 positive BMSC and were analyzed by live cell imaging. With and without cytochalasin B. (Scale bar, 10 µm.) (B) Multiple primary AML blasts were cultured on rLV.EF1. mCherry-Mito-9 Lentivirus transduced BMSC with and without cytochalasin B; the percentage of mCherry positive AML is shown. (C) AML blasts were stained with Vybrant Dil for 1 hour and washed 3 times in PBS. BMSC were stained with MitoTracker green FM for 1 hour and washed 3 times in PBS. AML blasts and BMSC were then cocultured for 24 hours before fixation, using paraformaldehyde. Cells were visualized by confocal microscopy.