Mitochondria acquired by the AML blasts are functionally active and contribute to the metabolic capacity. (A) Primary AML blasts were grown with and without BMSC for 72 hours and then analyzed independently, using the Seahorse XFp Analyzer with the Mito Stress Test Kit. Data represented as mean ± standard deviation. Sequential injections of Oligomycin (O), carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (F), and Rotenone (R) were used to obtain respiration dynamics presented in panel B. (C) Primary AML blasts were grown with and without BMSC, and after 72 hours, the ATP production capacity was analyzed by CellTitre-Glo, with cell numbers normalized. (D) BMSC were cultured with control KD AML or NOX2 KD AML for 72 hours. The blasts were then analyzed, using the Seahorse Extracellular Flux Analyzer; basal and maximum mitochondrial respiration is presented.