Figure 4.
![Figure 4. Intracellular cytokine staining of CD8+ DILs after restimulation with mature DCs alone (mDC) or WT1 mRNA-electroporated DCs (mDC WT1). The WT1-specific T-cell cytokine response was evaluated by comparing mDC WT1 with mDC (all patients examined: UPN03, 05, 06, 08, 14, 17, 21, 22, 29, 30, 34, 35, and 47; long-term [LT] survivors: UPN06, 08, 14, 17, 21, 22, 29, 34, and 35; non-LT survivors: UPN03, 05, 30, and 47). IFN-γ+, interleukin-5+ (IL-5+), or TNF-α+ CD8+ T cells are shown for all patients (A), LT survivors (B), and non-LT survivors (C). Polyfunctional TNF-α+/IFN-γ+ CD8+ T cells are shown in the same patients (D).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/130/15/10.1182_blood-2017-04-780155/4/blood780155f4.jpeg?Expires=1735841315&Signature=JU2OaFzCjf-u28BQlWa4gbNZavJCwTtT8p3ooekQAxdul4LNioYybqDux~eH~E~38vwaZTYju-9nZRo55Cnu178uVBAjeEJZfUIRs5W-fEOYJnUpE4~QHrqlzdgcbOM7Idrb4XOj76G96pbzRyH1Az6f94lamPk-gSp-AAKuuv3CkcZOIf8SA6zzTAO3QmBaRLPZIAeMoPXED6tsB7xX9wD2ZFBVcnDXKs9-Bh61M8CEPK7Oq7YnGx~Es2hmt2W7ee1OJaibJ2PpsbPU8UIwxHsyb0THOzIzpRBg81Xouvh7XZC~kStHSHAUGifQ4NrYy0BOJTyEWhNVQRj5WPOzLA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Intracellular cytokine staining of CD8+DILs after restimulation with mature DCs alone (mDC) or WT1 mRNA-electroporated DCs (mDC WT1). The WT1-specific T-cell cytokine response was evaluated by comparing mDC WT1 with mDC (all patients examined: UPN03, 05, 06, 08, 14, 17, 21, 22, 29, 30, 34, 35, and 47; long-term [LT] survivors: UPN06, 08, 14, 17, 21, 22, 29, 34, and 35; non-LT survivors: UPN03, 05, 30, and 47). IFN-γ+, interleukin-5+ (IL-5+), or TNF-α+ CD8+ T cells are shown for all patients (A), LT survivors (B), and non-LT survivors (C). Polyfunctional TNF-α+/IFN-γ+ CD8+ T cells are shown in the same patients (D).
