Increased CD11b fraction and circulating CD207⁺CD1a⁺myeloid cells in peripheral blood of patients with active LCH. (A) Gating strategy used to discriminate CD11b myeloid lineage and the CD4 T-cell compartment. (B-D) Independent data showing percentage of CD11b^highCD4^low; CD11b^lowCD4^neg, and CD11b^high plus CD11b^low cells in patients with AD (N = 9) compared with patients with NAD (N = 11) and healthy adults (N = 12). Unpaired 2-tailed t test was performed to compare AD patients with NAD patients or healthy adults. *P < .05; ***P < .001. (E) Representative dot plot and gating strategies used to discriminate CD11b and CD11c subpopulations from PBMCs. The expression of CD207 and CD1a was analyzed in CD11b^highCD11c⁺ monocytes, CD11b^lowCD11c^high DCs, and CD11b^lowCD11c^low cells. (F-H) Independent data for positive CD207 cells, including CD207⁺ plus CD207⁺CD1a⁺ (Q1+Q2), for each gated subpopulation in AD patients (N = 9), NAD patients (N = 10), and healthy adults (N = 9) are shown. A 2-tailed Mann-Whitney test was performed (**P < .01; ***P < .001).