ROS and NOXs regulate Mo-DC differentiation and maturation independently of NOX2. Freshly isolated Mos from healthy donors (A-C) (n = 10) or gp91phox/NOX2^−/− CGD patients (D-F) (n = 4) were treated with or without NAC or DPI at indicated concentrations for 15 minutes prior to adding GM-CSF/IL-4 differentiation stimuli or LPS maturation stimuli. Differentiation was carried out for 6 days and LPS maturation for 2 more days. On day 7 (immature Mo-DC) or day 9 (mature Mo-DC), cells were harvested and prepared for flow cytometry analysis after incubation with CD86 PE/CD209 PerCP-Cy5.5/CD83 APC cocktail antibodies for 20 minutes in the dark. Data are presented as mean fluorescence intensity (MFI) of CD209 (A and D), CD86 (B and F), and CD83 (C and E) and are expressed as mean ± standard error of the mean (SEM). One-way ANOVA with Tukey test *P < .05, **P < .01, and ***P < .001.