NOX5 regulates Mo-DC differentiation. Flow cytometry analysis of Mos treated with increasing concentration of (A) NAC (5-20 mM), (B) DPI (0.05-0.5 µM), (C) CEL (0.5-2 µM), and (D) EBS (10-40 µM) 15 minutes prior to addition of the cytokine differentiation stimuli (GM-CSF/IL-4) for 6 days. On day 7, cells were harvested and prepared for flow cytometry analysis after incubation with CD86 PE/CD209 PerCP-Cy5.5/CD83 APC cocktail antibodies for 20 minutes in the dark. (E) Mos treated for 18 hours with GM-CSF/IL-4 to induce NOX5 expression and then transfected with 200 nM scrambled or NOX5 siRNA. Five days later, cells were harvested and prepared for flow cytometry analysis as above. Data are presented as MFI of CD209 and are expressed as mean ± SEM (n = 4). (F) Representative western blot analysis of Mo-DC transfection and (G) relative quantification (n = 4, arbitrary units). One-way ANOVA with Tukey test. *P < .05, ***P < .001.