Protein and RNA levels of iron-related genes in liver and spleen of Pfn2^−/−and WT mice. Liver (A) ferritin L and ferritin H protein levels measured by western blotting are reduced in Pfn2^−/− mice compared with WT controls, whereas splenic (D) ferroportin protein levels are increased in Pfn2^−/− mice compared with WT controls. Ribosomal protein S6 levels were used for calibration. Graphs represent quantified data normalized to the WT sample (set as 100%). Samples size (n) is indicated. RNA expression of iron-related genes was analyzed by qPCR in liver (C) and spleen (E). mRNA expression was calibrated with Rpl19 and Tbp mRNA expression and normalized to WT mice (set as 100%). Sample size (n) is indicated. (B) Plasma levels of Il6 were measured by Luminex immunoassay. Bmp6, bone morphogenetic protein 6;Hamp1, hepcidin; Hif1α/2α, hypoxia inducible factor 1α/2α; Hmox1, heme oxygenase 1; Il1β/6, interleukin1β/6; Lcn2, lipocalin 2; Saa1, serum amyloid A1; Slc40a11, ferroportin (protein: Fpn1); Tnfα, tumor necrosis factorα. *P ≤ .05, **P ≤ .01.