The BMP coreceptor HJV is dispensable for FKBP12-dependent hepcidin activation. (A) Primary hepatocytes from Hjv KO mice were isolated and treated for 18 hours with rapamycin and Torin1 (RAPA 100 nM, red bar, and T1 100 nM, light red bar respectively), TAC (1 μg/mL, blue bar) and CA (1 μg/mL, light blue bar) and GPI-1046 (100 μg/mL, green bar). RNA was isolated and hepcidin (Hamp) levels measured by qRT-PCR. Hprt1 was used as housekeeping gene. Mean ΔCt values in each group have been subjected to a change of origin by subtracting the mean ΔCt of vehicle-treated hepatocytes [vehicle(1): 5.1; vehicle(2): 5.6; vehicle(3): 3.7]. (B) TAC-dependent Hamp increase was evaluated in WT and Hjv KO hepatocytes. Mean ΔCt values in each group were subjected to a change of origin by subtracting the mean ΔCt of vehicle-treated hepatocytes (WT, 1.7; Hjv KO, 4.1). A representative experiment, made in triplicate, is shown. (C) Primary hepatocytes from WT and Hjv KO mice were isolated and treated with increasing concentrations of TAC. Hamp ΔCt values in each group were subjected to a change of origin by subtracting the mean ΔCt of vehicle-treated WT hepatocytes, as in panel B. A representative experiment, made in triplicate, is shown. Error bars indicate SD. *P < .05; **P < .01; ***P < .001; ****P < .0001. Estimates of the fold changes in gene expression (2^−ΔΔCt) are shown in the graphs.