PAI-1 regulates mobilization of functional multipotent HSPCs. (A) Schema for mobilization experiments. (B) Representative flow cytometric profiles and MFI (n = 6) for MT1-MMP and CD44 expressions in LSK CD34⁻ cells. (C) Representative flow cytometric profiles of circulating LSK cells. (D-E) Percentages of mobilized LSK cells (D) and the number of colony-forming cells (E) in PB (n = 6). (F) Schema for long-term competitive reconstitution experiments. (G) Percentages of donor cells in PB of primary and secondary recipients at 12 weeks after transplantation. Pooled data (n = 5) of 3 independent experiments are shown. (H) Representative flow cytometric profiles of donor-derived multilineage reconstitution in primary recipients. Means ± SD are shown in each bar graph. CFU, colony-forming unit; MNC, mononuclear cell; NS, not significant; Tx, transplantation.