Cell kinetics in peripheral blood and bone marrow trafficking of WT1-specific TCR-T cells after the cell transfer into 8 patients. (A) Panels show kinetics of 3 patients who received 2 × 108 cells per infusion (cohort 1), 3 patients who received 1 × 109 cells (cohort 2), and 2 patients, TCR-WT-09-N05 and TCR-WT-10-F02, who received 9.7 × 108 cells (first cycle), 5.6 × 108 cells (second cycle), and 6.5 × 108 cells per infusion (extracohort), respectively. PB was collected at baseline and at predetermined times during a period of 58 days. DNA samples were extracted from the PBMCs, and TCR gene copy numbers were measured by qPCR. (B) Bone marrow trafficking of WT1-TCR-T cells in 3 patients compared with those in the peripheral blood at the same times is presented. The detection limit of the transduced cells is 100 copies/105 cells.
