Acetazolamide suppresses thrombus formation in vivo. Mice were administered acetazolamide (7 mg/kg) or vehicle by single bolus intravenous injection, followed immediately by DyLight 488–conjugated anti-GPIbβ antibody to label platelets. Carotid artery damage was achieved by treatment with FeCl3 as previously described.8 Fluorescently labeled platelets adhering at the site of injury could then be imaged continuously by intravital fluorescence microscopy. Images at frames indicated in panel A correspond to time points indicated in panel Bi, which shows median fluorescence intensity, quantified by using ImageJ. Analysis of the area under the curve for media fluorescence is shown in panel Bii as interleaved box plots with whiskers showing minimum to maximum values, median, and interquartile range. Data analysis was by Wilcoxon signed rank test, P < .05. *Considered significant. Scale bar, 500 µm. Data are from 8 pairs of mice. Reproduced with permission from Agbani et al.8
