Figure 3.
Figure 3. Genetic and immunohistochemical analyses of the PD-L1 and PD-L2 loci. (A) Location and color-labeling of the BAC clones on 9p24.1 used for FISH. RP11-599H20 including PD-L1, labeled red. RP11-635N21 including PD-L2, labeled green. (B) Representative images of FISH results for the different categories. PD-L1 in red, PD-L2 in green, fused (F) signals in yellow and centromeric probe (CEP9) in aqua (A). In these images, disomy reflects 2A:2F; polysomy, 3A:3F; copy gain, 3A:6F; and amplification, 15+F. (C) PD-L1 (brown)/PAX5 (red) immunohistochemistry in the cHL cases with 9p24.1 disomy, polysomy, copy gain, and amplification from panel B. Scale bar indicates 50 μm. Reprinted with permission © (2016) American Society of Clinical Oncology. All rights reserved. Roemer, MG et al: J Clin Oncol Vol. 34 (23), 2016 2690-7.

Genetic and immunohistochemical analyses of the PD-L1 and PD-L2 loci. (A) Location and color-labeling of the BAC clones on 9p24.1 used for FISH. RP11-599H20 including PD-L1, labeled red. RP11-635N21 including PD-L2, labeled green. (B) Representative images of FISH results for the different categories. PD-L1 in red, PD-L2 in green, fused (F) signals in yellow and centromeric probe (CEP9) in aqua (A). In these images, disomy reflects 2A:2F; polysomy, 3A:3F; copy gain, 3A:6F; and amplification, 15+F. (C) PD-L1 (brown)/PAX5 (red) immunohistochemistry in the cHL cases with 9p24.1 disomy, polysomy, copy gain, and amplification from panel B. Scale bar indicates 50 μm. Reprinted with permission © (2016) American Society of Clinical Oncology. All rights reserved. Roemer, MG et al: J Clin Oncol Vol. 34 (23), 2016 2690-7.

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