p66Shc deficiency results in an upregulation of ILT3 in the CLL-derived MEC-1 cell line. (A) Immunoblot analysis of p66Shc expression in MEC-pc and MEC-p66Shc cells. (B) Gene categories significantly enriched for genes differentially expressed in MEC-p66Shc vs MEC-pc cells (Fisher’s exact test, adjusted P < .05, dashed line). (C) qRT-PCR analysis of ILT3 expression (upper panel) and p66Shc expression (lower panel) in MEC-pc and MEC-p66Shc cells. Each dot represents an independent measurement. (D) Flow cytometric analysis of ILT3 surface expression on wild-type MEC, MEC-pc, and MEC-p66Shc cells. (E) qRT-PCR analysis of ILT3 gene expression (upper panel) and flow cytometric analysis of ILT3 surface expression (lower panel) on individual MEC-pc and MEC-p66Shc clones (n = 18 and 8, respectively). Shown are mean values ± SD. *P < .05; **P < .01; ***P < .001. MFI, mean fluorescence intensity.